Preparation of Double Antibody Radioimmunoassay for Determination of Alpha fetoprotein as a Tumor Marker using BALB/C Mice as Host Animals
- 1. Labelled compound Dep. Labs Center, Atomic Energy Authority, Cairo (Egypt)
Description
The aim of the present work was to prepare liquid phase radioimmunoassay system (RIA) reagents. Development as well as optimization and validation of this RIA system for the measurement of alpha fetoprotein (AFP) in human serum are described. The production of polyclonal antibodies was carried out by immunizing four BALB/C mice subcutaneously. The preparation of 125I-AFP tracer was performed using chloramine-T oxidation method. The preparation of AFP standards was done by diluting cord sera using assay buffer. The results obtained provide a highly sensitive,precise and accurate RIA system of AFP based on liquid phase separation. In conclusion, this assay could be used for the diagnosis and management of patients with certain malignant diseases and in prenatal diagnosis of neural tube defects
Additional details
Publishing Information
- Journal Title
- Arab Journal of Nuclear Sciences and Applications
- Journal Volume
- 42
- Journal Issue
- 3
- Journal Page Range
- p. 47-56
- ISSN
- 1110-0451
- CODEN
- AJNADV
INIS
- Country of Publication
- Egypt
- Country of Input or Organization
- Egypt
- INIS RN
- 40092914
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE; S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
- Descriptors DEI
- ACCURACY; ANIMALS; ANTIBODIES; BLOOD SERUM; CHEMICAL PREPARATION; DIAGNOSIS; GLYCOPROTEINS; IODINE 125; LIVER; MICE; MOLECULAR WEIGHT; NEOPLASMS; PATIENTS; RADIOACTIVITY; RADIOIMMUNOASSAY; SENSITIVITY
- Descriptors DEC
- ANIMALS; BETA DECAY RADIOISOTOPES; BIOASSAY; BIOLOGICAL MATERIALS; BLOOD; BLOOD PLASMA; BODY; BODY FLUIDS; CARBOHYDRATES; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DIGESTIVE SYSTEM; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; GLANDS; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOISOTOPES; RODENTS; SACCHARIDES; SYNTHESIS; TRACER TECHNIQUES; VERTEBRATES