A sensitive fluorescence detection strategy for H₂O₂ and glucose by using aminated Fe–Ni bimetallic MOF as fluorescent nanozyme

An aminated Fe–Ni bimetallic metal–organic framework (Fe₃Ni-MOF-NH₂) with both peroxidase-like activity and fluorescence properties was developed. Fe₃Ni-MOF-NH₂ possessed the enhanced peroxidase-like activity through the enhanced electron transfer process and hydroxyl radical (·OH) generation. It was found that the amino group endowed the material with fluorescent property and the metal site Ni in Fe₃Ni-MOF-NH₂ could also enhance the fluorescence emission intensity (Ex = 345 nm, Em = 452 nm). Based on the dual excellent performance of Fe₃Ni-MOF-NH₂, a novel sensitive fluorescence detection strategy for H₂O₂ and glucose was designed and achieved. First, Fe₃Ni-MOF-NH₂ converted H₂O₂ to ·OH by exerting peroxidase-like activity, and ·OH converts catechol to o-benzoquinone. Then, the amino group in Fe₃Ni-MOF-NH₂ connected to o-benzoquinone, which resulted in its fluorescence quenching. The detection limit of H₂O₂ was as low as 5 nM. Combined with glucose oxidase which can oxidize glucose and produce H₂O₂ the glucose could be indirectly determined with a detection limit of 40 nM. The method was applied to the detection of low-level glucose in human urine samples with good recoveries and reproducibilities. Graphical abstract