The broth effect and mutation frequency decline in cells of Escherichia coli after irradiation with UV-light

Dependence of the broth effect and the phenomenon of mutation frequency decline on the dose of the applied UV radiation was investigated in the strain Escherichia coli B/r Hcr+ thy trp. Reversions to Trp+ were followed. The degree of the broth effect and the mutation frequency decline is minimal within the range of UV doses corresponding to a survival of cells lower than 10-1. In connection with the two effects, excision of thymine dimers, initiation of synthesis, synthesis and degradation of DNA were also investigated. It was found that the stimulation and the inhibition of an inaccurate postreplication repair mechanism, rather than the inhibition and the stimulation of excision of thymine dimers, are responsible for the broth effect and the mutation frequency decline, respectively. (author)