Published March 1984
| Version v1
Journal article
The preparation of radioactivity labelled DNA
- 1. Sussex Univ., Brighton (UK). Safety and Radiation Protection Service
- 2. Medical Research Council, Brighton (UK). Cell Mutation Unit
- 3. Sussex Univ., Brighton (UK). School of Biological Sciences
Description
The nick translation reaction, which uses the endonuclease enzyme to incorporate pieces of DNA into the genetic material of other organisms, is being used more and more in Molecular Biology research for the preparation of pure DNA labelled with 32P. However results are presented which show that high radiation doses are received by the hands of nick translation workers. A Scheme of Work to reduce these doses is described. (author)
Additional details
Additional titles
- Augmented title (English)
- "3"2P-DNA, radiation protection
Publishing Information
- Journal Title
- Saf. Pract.
- Journal Volume
- 2
- Journal Issue
- 3
- Series
- Saf. Pract.
- Journal Page Range
- 25-26
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- United Kingdom
- INIS RN
- 15046545
- Subject category
- S61: RADIATION PROTECTION AND DOSIMETRY;
- Descriptors DEI
- DNA; HANDS; LABELLED COMPOUNDS; OCCUPATIONAL SAFETY; PERSONNEL DOSIMETRY; PHOSPHORUS 32; RADIATION DOSES; RADIATION PROTECTION; RECOMMENDATIONS; SAMPLE PREPARATION
- Descriptors DEC
- ARMS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; BODY AREAS; DAYS LIVING RADIOISOTOPES; DOSIMETRY; ISOTOPES; LIGHT NUCLEI; LIMBS; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; RADIOISOTOPES; SAFETY