Light scattering of a Bessel beam by a nucleated biological cell: An eccentric sphere model

Highlights: • Analytical solution for the scattering of a Bessel beam by an eccentric stratified sphere is presented. • Beam shape coefficients for an arbitrarily incident Bessel beam is analyzed. • Light scattering properties of a nucleated cell illuminated by a Bessel beam are analyzed. Within the framework of generalized Lorenz–Mie theory (GLMT), an eccentrically stratified dielectric sphere model illuminated by an arbitrarily incident Bessel beam is applied to investigate the scattering characteristics of a single nucleated biological cell. The Bessel beam propagating in an arbitrary direction is expanded in terms of vector spherical wave functions (VSWFs), where the beam shape coefficients (BSCs) are calculated rigorously in a closed analytical form. The effects of the half-cone angle of Bessel beam, the location of the particle in the beam, the size ratio of nucleus to cell, and the location of the nucleus inside the cell on the scattering properties of a nucleated cell are analyzed. The results provide useful references for optical diagnostic and imaging of particle having nucleated structure.