Published July 1987
| Version v1
Journal article
Identification of Lotus rhizobia by direct DNA hybridization of crushed root nodules
Creators
- 1. Dept. of Agriculture for Northern Ireland, Belfast
Description
Hybridization of crushed Lotus pedunculatus root nodules with 32P-labeled total genomic DNA probes was used to identify Rhizobium loti and Bradyrhizobium sp. (Lotus rhizobia). Probes always hybridized with homologous target DNA and frequency with DNAs of other strains from the same genus. Intergeneric hybridization did not occur. Results were comparable to those from colony hybridization
Additional details
Publishing Information
- Journal Title
- Appl. Environ. Microbiol.
- Journal Volume
- 53
- Journal Issue
- 7
- Series
- Appl. Environ. Microbiol.
- Journal Page Range
- 1705-1707
- ISSN
- 0099-2240
- CODEN
- AEMID
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 19022101
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BACTERIA; DNA; HYBRIDIZATION; PHOSPHORUS 32; ROOTS; TAXONOMY; TRACER TECHNIQUES
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; RADIOISOTOPES