Effect of Gamma-rays on In Vitro Culture of Mungbean Cotyledon

Multiple shoots were obtained from cotyledons of 7 mungbean cultivars cultured on B5 supplemented with 2, 4, 6 and 8 mg/1 BA. Each variety responded to BA differently. The medium containing 5 mg/1 BA was the best for multiple shoot induction. Cotyledons from 3 day-old-seeding gave the highest percentage of shoot multiplication (88.00 per mille) within 18 days. The best medium for root induction was M S medium supplemented with 1 g/1 K NO3 and 0.203 mg/1 IBA. Seeds of 3 mungbean cultivars were treated with gamma radiation at 200, 400, 600 and 800 Gy and cotyledons were cultured on the medium B5 containing 4 mg/1 BA to produce multiple shoots. Shoot and root formations were affected by radiation dose. At 800 Gy, shoot formation was completely inhibited. Rootings were accomplished at radiation dose as high as 400 Gy