Comparison of DNA double-strand breaks induced by 16O8+ in deterpenized DNA and intact cells
- 1. Academia Sinica, Lanzhou (China). Inst. of Modern Physics
- 2. Lanzhou Univ. (China). Dept. of Biology
Description
The yield of DNA double-strand breaks (DSBs) is sure to be influenced by the environment around DNA molecule. Inverse pulsed-field gel electrophoresis (PIGE) has been applied to compare the sensitivity of B16 cells and their DNA in DSBs induced by 75 MeV/u 16O8+ beam. Results show that the percentages of DNA released from the plug (PR) in both kinds of the samples increase with the dose and approach a similar quasi-threshold of about 81%. A simple new equation was presented to calculate the break level of DNA molecules. Within a certain dose, the relationship between the break level and the dose is linear. The yield of DSBs in deproteinized DNA was 1.11 DSBs/100 Mbp/Gy, while that in intact cells was 0.60 DSBs/100Mbp/Gy. It is testified that deproteinized DNA is more sensitive to oxygen ions irradiation than intact cells
Additional details
Publishing Information
- Journal Title
- Nuclear Science and Techniques
- Journal Volume
- 9
- Journal Issue
- 4
- Journal Page Range
- p. 235-238
- ISSN
- 1001-8042
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 30055259
- Subject category
- S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
- Descriptors DEI
- CHEMICAL RADIATION EFFECTS; DNA; DOSE-RESPONSE RELATIONSHIPS; ELECTROPHORESIS; GELS; MEV RANGE 10-100; OXYGEN 16; OXYGEN IONS; STRAND BREAKS
- Descriptors DEC
- CHARGED PARTICLES; COLLOIDS; DISPERSIONS; DNA DAMAGES; ENERGY RANGE; EVEN-EVEN NUCLEI; IONS; ISOTOPES; LIGHT NUCLEI; MEV RANGE; NUCLEI; NUCLEIC ACIDS; ORGANIC COMPOUNDS; OXYGEN ISOTOPES; RADIATION EFFECTS; SEPARATION PROCESSES; STABLE ISOTOPES