Biological effects induced by the inner-target reaction of accelerated 7Li+3 ions with wheat embryo

Using the mechanism of the nuclear reaction of accelerated 7Li+3 ions with the inner target in mutant material i.e 1H(7Li, 7Be)n, the biological effects were studied. The wheat seeds were irradiated with the doses ranged from 1.416 x 1010 ions/cm2 to 1.416 x 1012 ions/cm2. It was found that the cell membrane ruptured, the plasmolysis occurred, the nucleus shape changed. The serious changes of the chloroplast were as follows: the membrane protuberance, the grand disorder, the membrane disappearance, crista of mitochondrion rupture etc. by checking of the sub-microstructure of leaf cell. The single micronucleus and multi-micronucleus were observed at the interphase. The chromosome aberrational cells including chromosome fragment, lagging chromosome, chromosome bridge and circular chromosome were found during the mitosis. RAPD analysis of seedling genomic DNA variation in M2 generation of three mutants showed their DNA sequences had changed. The result confirmed that the implantation of 7Li+3 ions could induce genetic mutation in wheat