Induced mutation for bacterial blight resistance in mulberry

Buds of mulberry varieties Phai, Noi and SK2502 were irradiated by gamma radiation and then cultured on the Murashige and Skoog medium containing 1.0 mg/l of BA and Casein hydrolysate 1g/l. After proliferation of shoots, they were transferred to the rooting medium (MS + NAA 0.2 mg/l + IBA 0.2 mg/l). Plantlets of mulberry var. Noi were transplanted to soil in greenhouse for screening for bacterial blight disease resistance. All plants showed symptoms of disease. In vitro inoculation of the pathogen, Pseudomonas syringae p.v. mori, on plantlets of the mulberry var. Noi and Phai was conducted. All plants showed symptoms of the disease and died. In vitro screening will be continued with much larger populations in order to select the resistance traits. (author). 4 refs, 4 tabs