Methodology study on isotopic tracing HSA-IFN-α2b in vivo
Creators
- 1. The Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Inst. of Nuclear Medicine, Wuxi (China)
- 2. Wolwo Biological Technology Co., Ltd., Shanghai (China)
Description
125I-HSA-IFN-α2b was prepared with the method of CH-T. The labelling yields was 82.72%, radiochemical purity was more than 95% and the radioactivity was 0.26 TBq/g. The activity of antivirus of 125I-HSA-IFN-α2b was compared with that of HSA-IFN-α2b by WISH/VSV system in vitro. The results showed that bioactivity of HSA-IFN-α2b had no change before and after being labelled.The serum recovery 102.0% and organ recovery 104.0%(heart), 100.4%(lung), 100.9%(liver), 104.1%(spleen), 101.4%(kidney), 103.0%(small intestine)of 125I-HSA-IFN-α2b met the requirements. The results of the interference experiments showed that there was no interference on the determination. There- fore, 125I-HSA-IFN-α2b can be used in studying biodistribution and excretion of HSA-IFN-α2b. (authors)
Additional details
Publishing Information
- Journal Title
- Journal of Isotopes
- Journal Volume
- 21
- Journal Issue
- 4
- Journal Page Range
- p. 220-225
- ISSN
- 1000-7512
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 42073155
- Subject category
- S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY; S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- ACTIVITY LEVELS; ALBUMINS; BLOOD SERUM; CHEMICAL PREPARATION; CHEMICAL REACTION YIELD; EXCRETION; HEART; IMPURITIES; IN VITRO; IN VIVO; INTERFERENCE; INTERFERON; IODINE 125; KIDNEYS; LABELLED COMPOUNDS; LABELLING; LIVER; LUNGS; RADIOACTIVITY; SMALL INTESTINE; SPLEEN; TRACER TECHNIQUES
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD PLASMA; BODY; BODY FLUIDS; CARDIOVASCULAR SYSTEM; CLEARANCE; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; ELECTRON CAPTURE RADIOISOTOPES; GASTROINTESTINAL TRACT; GLANDS; GROWTH FACTORS; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; INTESTINES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LYMPHOKINES; MATERIALS; MITOGENS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; RESPIRATORY SYSTEM; SYNTHESIS; YIELDS
Optional Information
- Notes
- 14 figs., 2 tabs., 6 refs.