High levels of fluctuating asymmetry in populations of Apodemus flavicollis from the most contaminated areas in Chernobyl

Random deviations from the perfect symmetry of normally bilaterally symmetrical characters for an individual with a given genotype occur during individual development due to the influence of multiple environmental factors. Fluctuating asymmetry (FA) is often used as a measure of developmental instability, and can be estimated as the variance of the distribution of differences between the left and right sides. We addressed the question of whether levels of FA were elevated in radioactively contaminated populations living around Chernobyl compared to those in reference populations of the yellow-necked mouse (Apodemus flavicollis). In addition, we studied amounts of directional asymmetry (DA) when one side is larger than the other on average. There was a significant difference among populations, including reference populations, in the amount of both FA and DA. A higher level of FA was documented for the contaminated populations in close proximity to the failed Chernobyl reactor for both the asymmetry of size and shape. The FAs of size and shape were highest in populations from the most contaminated locations in the Chernobyl exclusion zone. Although the directional asymmetry of shape was also highest in the contaminated populations, it was not significantly different from those in most of the reference populations. Populations from less contaminated areas inside the Chernobyl exclusion zone did not express FA values different from those of the reference populations outside the affected area. FA of skulls of A. flavicollis may indicate the degree to which the level of radioactive contamination affects the development of animals at Chernobyl. However, the mechanisms leading to these effects are not clear and probably vary from population to population. There were significant correlations between the overall right to left differences for the Procrustes aligned shape configurations, centroid sizes, and intramuscular 137Cs. Detectable effects of radiation on developmental stability probably start to occur between 0.132 and 0.297 μGy/h