Published 2022 | Version v1
Journal article Metadata-only

New Analytical Method and its Validation to Determine Ceftriaxone Sodium by RP-HPLC

Creators

  • 1. Damascus Univ., Faculty of Sciences, Damascus (Syrian Arab Republic) Dept. of Chemistry
  • 2. Research Institute for Pharmaceutical and Chemical Industries, Scientific Studies and Research Centre (SSRC),Damascus (Syrian Arab Republic)

Description

The concentration of Ceftriaxone Sodium was determined by using Reversed Phase Liquid Chromatography (RP-HPLC) equipped with UV detector on wavelength of 254 nm and column C18 (25cm, 4.6mm,5μm). The mobile phase consists of (Buffer 93%: 2-Propanol 7%) and the buffer is a solution of 5.1 g/L of Sodium Acetate Trihydrate adjusted to pH=6 with glacial acetic acid. The flow rate adjusted to 1.2 mL/min. Results were linear in the range of (8 - 178) μg/mL with correlation coefficient R2=0.9999. The relative standard deviation of (n=6) replicates was RSD%=0.74, This method was characterized by high precision, high specificity, low cost and suitable for quality control in pharmaceutical industries. (author)

Additional details

Publishing Information

Journal Title
Damascus University Journal for the Basic Sciences
Journal Volume
38
Journal Issue
2
Journal Page Range
p. 219-239
ISSN
1726-5487

INIS

Country of Publication
Syrian Arab Republic
Country of Input or Organization
Syrian Arab Republic
INIS RN
54127189
Subject category
S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
Resource subtype / Literary indicator
Numerical Data
Descriptors DEI
CHEMICAL ANALYSIS; CORRELATIONS; EXPERIMENTAL DATA; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; SODIUM; ULTRAVIOLET RADIATION; VALIDATION
Descriptors DEC
ALKALI METALS; CHROMATOGRAPHY; DATA; ELECTROMAGNETIC RADIATION; ELEMENTS; INFORMATION; LIQUID COLUMN CHROMATOGRAPHY; METALS; NUMERICAL DATA; RADIATIONS; SEPARATION PROCESSES; TESTING

Optional Information

Notes
26 refs., 9 figs., 6 tabs.