Radiation pre-treating straw hydrolyzed by cellulase resulted from immobilized Trichoderma reesei growing cells

Wheat and rice straw was irradiated by electron beam with different dose at the presence of 4% NaOH or without 4% NaOH. The powder fraction above 200 meshes in pretreated rice straw increased with increasing doses and it was more at presence of 4% NaOH than that without 4% NaOH. The pretreated straw was hydrolyzed with 1% cellulase at 40 degree C for 48 h. The glucose yield (%) was given a rise with the increase of irradiation dose and it was 70% and 80% over that of un-pretreated rice and wheat straw, respectively. At the presence of 4% NaOH, the glucose yield increased as the irradiation dose increased from 0 to 5 x 105 Gy, reaching a maximum, 35% for wheat straw and 36.6% for rice straw, which increased by about 2.5 times in comparison with un-pretreated straw, then decrease with increasing the irradiation dose to 10 x 105 Gy. The glucose yield reached 19% and 22% for rice and wheat straw in 6 days of hydrolysis, respectively