Use of 32P in the study of phosphorus uptake and metabolism in plants

The method allows following the uptake and metabolism of phosphorus and isolating in acids both soluble organic phosphorus compounds (i.e., esters of phosphoric acid with glycides, free nucleotides, inorganic phosphorus) and insoluble phosphorus compounds (i.e., macromolecular compounds, phospholipids). The method of separating low-molecular and acid-soluble phosphorus compounds by one-dimensional paper chromatography was also tested. In maize roots the following low-molecular phosphorus compounds were determined by autoradiochromatography: ATP, ADP, G-1-P, AMP, G-6-P, di PGA, Ri-5-P, F-1, 6-diP, 3-PGA, Pan. (author)