Optimization of 13C direct detection NMR methods

13C-detected experiments are still limited by their inherently lower sensitivity, as compared to the equivalent 1H-detected experiments. Improving the sensitivity of 13C detection methods remains a significant area of NMR research that may provide better means for studying large macromolecular systems by NMR. In this communication, we show that 13C-detected experiments are less sensitive to the salt concentration of the sample solution than 1H-detected experiments. In addition, acquisition can be started with anti-phase coherence, resulting in higher sensitivity due to the elimination of the final INEPT transfer step