Published November 4, 2006 | Version v1
Journal article Metadata-only

Crystallization and preliminary X-ray diffraction studies of the terminal oxygenase component of carbazole 1,9a-dioxygenase from Nocardioides aromaticivorans IC177

Creators

  • 1. Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan)
  • 2. Professional Programme for Agricultural Bioinformatics, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan)
  • 3. Department of Biochemistry, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602 (Japan)

Description

The terminal oxygenase component of carbazole 1,9a-dioxygenase from N. aromaticivorans IC177 was crystallized and diffraction data were collected to 2.30 Å resolution. Carbazole 1,9a-dioxygenase (CARDO) catalyzes the dihydroxylation of carbazole by angular-position (C9a) carbon bonding to the imino nitrogen and its adjacent C1 carbon. CARDO consists of a terminal oxygenase component and two electron-transfer components: ferredoxin and ferredoxin reductase. The terminal oxygenase component (43.9 kDa) of carbazole 1,9a-dioxygenase from Nocardioides aromaticivorans IC177 was crystallized at 293 K using the hanging-drop vapour-diffusion method with PEG 8000 as the precipitant. The crystals diffract to 2.3 Å resolution and belong to space group C2

Availability note (English)

Available from http://dx.doi.org/10.1107/S1744309106044939; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225353

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225353;
DOI
10.1107/S1744309106044939;
PII
S1744309106044939;

Publishing Information

Journal Title
Acta Crystallographica. Section F
Journal Volume
62
Journal Issue
Pt 12
Journal Page Range
p. 1212-1214
ISSN
1744-3091
CODEN
ACSFCL

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46061579
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
BONDING; CARBON; CRYSTALLIZATION; CRYSTALS; DIFFUSION; ELECTRON TRANSFER; NITROGEN; OXYGEN; RESOLUTION; SPACE GROUPS; X-RAY DIFFRACTION
Descriptors DEC
COHERENT SCATTERING; DIFFRACTION; ELEMENTS; FABRICATION; JOINING; NONMETALS; PHASE TRANSFORMATIONS; SCATTERING; SYMMETRY GROUPS

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2006
Notes
PMCID: PMC2225353; PMID: 17142899; PUBLISHER-ID: bw5172; OAI: oai:pubmedcentral.nih.gov:2225353