Effect of UVB irradiation on JAK-STAT pathway and IL-6, L-8 in Human umbilical vein endothelial cells

Human umbilical vein endothelial cells (HUVECs) were irradiated with different doses (10, 20 and 40 J/m²) to investigate the role of JAK-STAT pathway and IL-6, IL-8 in UVB irradiation. After 24 h, the expression level of IL-6 mRNA and IL-8 mRNA was detected by real-time fluorescent quantitative PCR, and that of JAK1, JAK2, STAT1, STAT3, p-JAK1, p-JAK2, p-STAT1 and p-STAT3 was measured by Western-blot. Compared with the control group, the expression level of IL-6 mRNA and IL-8 mRNA increased, and that of JAK1, STAT1, p-JAK1, p-STAT1, p-JAK2 and p-STAT3 increased with the dose of UVB, except JAK2 and STAT3. It can be concluded that JAK-STAT pathway and IL-6 together with IL-8 play a role in HUVECs with UVB irradiation. (authors)