Published 1985
| Version v1
Journal article
ADP-ribosylation of membrane components by pertussis and cholera toxin
Description
Pertussis and cholera toxins are important tools to investigate functional and structural aspects of the stimulatory (N/sub s/) and inhibitory (N/sub i/) regulatory components of adenylyl cyclase. Cholera toxin acts on N/sub s/ by ADP-ribosylating its α/sub s/ subunit; pertussis toxin acts on N/sub i/ by ADP-ribosylating its α; subunit. By using [32P]NAD+ and determining the transfer of its [32P]ADP-ribose moiety to membrane components, it is possible to obtain information on N/sub s/ and N/sub i/. A set of protocols is presented that can be used to study simultaneously and comparatively the susceptibility of N/sub s/ and N/sub i/ to be ADP-ribosylated by cholera and pertussis toxin
Additional details
Publishing Information
- Journal Title
- Methods Enzymol.
- Journal Volume
- 109
- Series
- Methods Enzymol.
- Journal Page Range
- 566-571
- ISSN
- 0076-6879
- CODEN
- MENZA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 18051043
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Resource subtype / Literary indicator
- Numerical Data
- Descriptors DEI
- ADP; BIOCHEMICAL REACTION KINETICS; CELL MEMBRANES; CHOLERA; CYCLASES; ENZYME ACTIVITY; EXPERIMENTAL DATA; LABELLED COMPOUNDS; NAD; PHOSPHORUS 32; RIBOSE; TOXINS; TRACER TECHNIQUES
- Descriptors DEC
- ALDEHYDES; ANTIGENS; BACTERIAL DISEASES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOHYDRATES; CELL CONSTITUENTS; COENZYMES; DATA; DAYS LIVING RADIOISOTOPES; DISEASES; ENZYMES; INFECTIOUS DISEASES; INFORMATION; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIGHT NUCLEI; LYASES; MEMBRANES; MONOSACCHARIDES; NUCLEI; NUCLEOTIDES; NUMERICAL DATA; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PENTOSES; PHOSPHORUS ISOTOPES; RADIOISOTOPES; REACTION KINETICS; SACCHARIDES