Published July 1992 | Version v1
Report Metadata-only

Detection of cells infected by Thailand strains of pseudorabies virus using a specific DNA probe

Creators

  • 1. Chulalongkorn Univ., Bangkok (Thailand). Dept. of Pathology
  • 2. Mahidol Univ., Bangkok (Thailand). Dept. of Biochemistry
  • 3. Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Unit of Molecular Virology

Description

The Thailand strains of pseudorabies virus (PRV) have been shown to belong to the type IV group based on the Bam HI restriction enzyme pattern of PRV DNA. This pattern was only found in isolates originating from Thailand. A DNA probe (pTM16) cloned from the Phylaxia strain of PRV hybridized to both the Thailand PRV isolates CB-1 and NB-1 and defines also the difference in the Bam H1 restriction enzyme pattern. This DNA probe can thus not only be used in the diagnosis of PRV in Thailand but also in combination with Bam HI cleavage to discriminate between the European and Thailand strains of PRV. A similar sensitivity of detection was found both in the European and Thailand strains of PRV. (author). 14 refs, 3 figs

Part of:
Regional network for Latin America on animal disease diagnosis using immunoassay and labelled DNA probe techniques

Additional details

Publishing Information

Imprint Title
Regional network for Latin America on animal disease diagnosis using immunoassay and labelled DNA probe techniques
Imprint Pagination
196 p.
Journal Page Range
p. 77-84.
ISSN
1011-4289
Report number
IAEA-TECDOC--657

Conference

Title
Final research co-ordination meeting on regional network for Latin America on animal disease diagnosis using immunoassay and labelled DNA probe techniques.
Dates
22-26 Oct 1990.
Place
Heredia (Costa Rica).

INIS

Country of Publication
International Atomic Energy Agency (IAEA)
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
23066873
Subject category
S60: APPLIED LIFE SCIENCES;
Resource subtype / Literary indicator
Conference
Descriptors DEI
DNA; NUCLEIC ACID HYBRIDIZATION; SENSITIVITY; SPECIFICITY; SWINE; VIRAL DISEASES; VIRUSES
Descriptors DEC
ANIMALS; BIOTECHNOLOGY; DISEASES; DOMESTIC ANIMALS; GENETIC ENGINEERING; INFECTIOUS DISEASES; MAMMALS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PARASITES; VERTEBRATES

Optional Information