Effect of hydrogen peroxide and cyclophosphamide on the proliferation of splenic lymphocytes in irradiated mice

Objective: To study the effect of chemical factors on the proliferation of splenic lymphocytes in irradiated mice. Methods: To investigate the proliferation of splenic lymphocytes using 3H-TdR incorporation. Results: The hydrogen Peroxide (10-6 mol/L and 10-5 mol/L) increased significantly the proliferation of splenic lymphocytes induced by Con A (concanavalin A) when hydrogen Peroxide and Con A were simultaneously added into splenocytes in mice. The proliferation of splenic lymphocytes was decreased significantly to add the hydrogen Peroxide at 56 h later. It was lower than control induced by LPS (lipopolysaccharide) and Con A When the concentration of CP (cyclophosphamide) was ≥10-4 mol/L(LPS group) and ≥10-3 mol/L (Con A group). It was also decreased significantly when the concentration of hydrogen Peroxide was ≥10-6 mol/L and the concentration of CP was ≥10-3 mol/L in irradiated mice. Conclusion: Low dose hydrogen Peroxide may induce the adaptive response and hydrogen Peroxide and CP could not induce the resistance to irradiation on the proliferation of splenic lymphocytes in mice