Microarray multiplex assay for the simultaneous detection and discrimination of hepatitis B, hepatitis C, and human immunodeficiency type-1 viruses in human blood samples
Creators
- 1. Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Review, Food and Drug Administration, Bethesda, MD 20892 (United States)
- 2. Division of Viral Products, Center for Biologics Evaluation and Review, Food and Drug Administration, Bethesda, MD 20892 (United States)
- 3. Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Review, Food and Drug Administration, Bethesda, MD 20892 (United States)
Description
Hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type-1 (HIV-1) are transfusion-transmitted human pathogens that have a major impact on blood safety and public health worldwide. We developed a microarray multiplex assay for the simultaneous detection and discrimination of these three viruses. The microarray consists of 16 oligonucleotide probes, immobilized on a silylated glass slide. Amplicons from multiplex PCR were labeled with Cy-5 and hybridized to the microarray. The assay detected 1 International Unit (IU), 10 IU, 20 IU of HBV, HCV, and HIV-1, respectively, in a single multiplex reaction. The assay also detected and discriminated the presence of two or three of these viruses in a single sample. Our data represent a proof-of-concept for the possible use of highly sensitive multiplex microarray assay to screen and confirm the presence of these viruses in blood donors and patients
Additional details
Identifiers
- DOI
- 10.1016/j.bbrc.2007.03.087;
- PII
- S0006-291X(07)00577-3;
Publishing Information
- Journal Title
- Biochemical and Biophysical Research Communications
- Journal Volume
- 356
- Journal Issue
- 4
- Journal Page Range
- p. 1017-1023
- ISSN
- 0006-291X
- CODEN
- BBRCA9
INIS
- Country of Publication
- United States
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 39014737
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- AIDS VIRUS; BLOOD; GLASS; HEPATITIS; MICROARRAY TECHNOLOGY; OLIGONUCLEOTIDES; PATHOGENS; PATIENTS; POLYMERASE CHAIN REACTION; PUBLIC HEALTH; TRANSFUSIONS
- Descriptors DEC
- BIOLOGICAL MATERIALS; BIOTECHNOLOGY; BODY FLUIDS; DIGESTIVE SYSTEM DISEASES; DISEASES; DNA; GENE AMPLIFICATION; MATERIALS; MEDICINE; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PARASITES; THERAPY; VIRUSES
Optional Information
- Copyright
- Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.