Published June 25, 2010 | Version v1
Journal article Metadata-only

Gold-silver-alloy nanoprobes for one-pot multiplex DNA detection

Creators

  • 1. Centro de Investigacao em Genetica Molecular Humana (CIGMH), Departamento de Ciencias da Vida, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica (Portugal)
  • 2. Rede de Quimica e Tecnologia (REQUIMTE), Departamento de Quimica, Faculdade de Ciencias, Universidade do Porto, 4169-007 Porto (Portugal)
  • 3. Rede de Quimica e Tecnologia (REQUIMTE), Departamento de Quimica, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica (Portugal)

Description

A specific colorimetric DNA detection method based on oligonucleotide functionalized gold-silver-alloy nanoparticles (AuAg-alloy-nanoprobes) is presented. The AuAg-alloy-nanoprobes were then used for the specific detection of a DNA sequence from TP53-a gene involved in cancer development. The AuAg-alloy-nanoprobes were then used in combination with Au-nanoprobes for a one-pot dual-colour detection strategy that allowed for the simultaneous differential detection of two distinct target sequences. This system poses an unprecedented opportunity to explore the combined use of metal nanoparticles with different composition towards the development of a multiplex one-pot colorimetric assay for DNA detection.

Availability note (English)

Available from http://dx.doi.org/10.1088/0957-4484/21/25/255101

Additional details

Identifiers

DOI
10.1088/0957-4484/21/25/255101;
PII
S0957-4484(10)51134-6;

Publishing Information

Journal Title
Nanotechnology (Print)
Journal Volume
21
Journal Issue
25
Journal Page Range
[5 p.]
ISSN
0957-4484

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
43025135
Subject category
S77: NANOSCIENCE AND NANOTECHNOLOGY;
Descriptors DEI
COLOR; DETECTION; DNA SEQUENCING; GENES; GOLD; NANOSTRUCTURES; NEOPLASMS; OLIGONUCLEOTIDES; PARTICLES; SILVER ALLOYS
Descriptors DEC
ALLOYS; DISEASES; DNA; ELEMENTS; METALS; NUCLEIC ACIDS; OPTICAL PROPERTIES; ORGANIC COMPOUNDS; ORGANOLEPTIC PROPERTIES; PHYSICAL PROPERTIES; STRUCTURAL CHEMICAL ANALYSIS; TRANSITION ELEMENT ALLOYS; TRANSITION ELEMENTS