Studies on the biological behaviour of uranium-plutonium mixed oxide aerosols: Inhalation experiments with rats and in vitro studies with alveolar macrophages
Creators
- 1. Kernforschungszentrum Karlsruhe G.m.b.H. (Germany, F.R.). Inst. fuer Genetik und Toxikologie von Spaltstoffen
- 2. Commission of the European Communities, Karlsruhe (Germany, F.R.). Gemeinsame Forschungsstelle - Europaeisches Inst. fuer Transurane
Description
The retention of spherical and of irregularly shaped (U, Pu) mixed oxides in rat lung was analyzed after inhalation and intratracheal instillation. Their biological behaviour was relatively independent of particle shape and application route with only a few percent of radioactivity being transferred to other organs. In vivo and in vitro uptake and intracellular distribution in rat and bovine alveolar macrophages were analyzed as dependent on various parameters. In addition, detailed electron microscopic studies were performed demonstrating particles within membrane limited vacuoles as well as lying free in the cytoplasm. Under in vitro conditions the uptake process was finished after a few hours. After differential centrifugation of lung or macrophage homogenates the particles sedimented in the first (1000 g) fraction. (orig.)
Availability note (English)
MF available from INIS under the Report Number.Abstract (German)
Der Einfluss der Partikelform auf das biologische Verhalten von (U, Pu)-Mischoxidpartikeln nach Inhalation oder nach intratrachealer Instillation wurde vergleichend untersucht. Das biologische Verhalten der Teilchen in der Rattenlunge war weitgehend unabhaengig von der Teilchenform und der Applikationsart. Die Translokation und Radioaktivitaet in andere Organe war minimal. Die Aufnahme und intrazellulaere Verteilung der Partikel in Rattenmakrophagen in vivo sowie in Rinderalveolarmakrophagen in vitro wurde in Abhaengigkeit von verschiedenen Parametern untersucht und elektronenmikroskopisch charakterisiert; die Partikel wurden teils frei im Cytoplasma, teils in Phago(-lyso-)somen nachgewiesen. Unter in vitro-Bedingungen war die Partikelaufnahme durch Rinderalveolarmakrophagen innerhalb weniger Stunden abgeschlossen. Nach differentieller Zentrifugation von Lungen- und Makrophagenhomogenaten sedimentierten die Partikel ueberwiegend in der ersten (1000 g) Fraktion. (orig.)
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Additional details
Additional titles
- Original title (German)
- Zum biologischen Verhalten von Uran-Plutonium-Mischoxidaerosolen: Inhalationsversuche mit Ratten und in vitro-Studien mit Alveolarmakrophagen
Publishing Information
- Imprint Pagination
- 212 p.
- Report number
- KFK--4172
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 18050836
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- CELL CONSTITUENTS; CENTRIFUGATION; IN VITRO; IN VIVO; INHALATION; INTRATRACHEAL ADMINISTRATION; LUNGS; MACROPHAGES; PLUTONIUM OXIDES; RADIONUCLIDE KINETICS; RATS; TRANSLOCATION; UPTAKE; URANIUM OXIDES
- Descriptors DEC
- ACTINIDE COMPOUNDS; ANIMAL CELLS; ANIMALS; BODY; CHALCOGENIDES; CONNECTIVE TISSUE CELLS; INTAKE; KINETICS; MAMMALS; ORGANS; OXIDES; OXYGEN COMPOUNDS; PHAGOCYTES; PLUTONIUM COMPOUNDS; RESPIRATORY SYSTEM; RODENTS; SEPARATION PROCESSES; SOMATIC CELLS; TRANSURANIUM COMPOUNDS; URANIUM COMPOUNDS; VERTEBRATES
Optional Information
- Secondary number(s)
- EUR--10846-DE.