Biomonitoring of uranium and heavy metal pollution

New protein was induced by intraperitoneal injection of uranium. It was purified from liver of carp to which uranium had been at administered 1.5mg/kg fresh for 6 days. The protein was separated by sephadex G-100 and AEAE-Sepharose and characteristics of the metallothionein were not shown by it. It has a molecular weight of 40 KD. Cytosol type superoxide dismutase in carp testis has at least three electrophoretically different forms. Cyanide inhibits the activity of cytosol type superoxide dismutase. When it stored in solution, superoxide dismutase changed into polymeric form which retain its activity. It has been purified by gel permeation, chloroform-ethanol precipitation, and ion exchange column chromatography. (Author)