Published October 1, 1984
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Development of a coupled method for demonstrating sister chromatid exchanges and chromosomal marking: application to the detection of radiation-induced chromosomal damage in human lymphocytes
Creators
- Al-Achkar, Walid
- Universite Pierre et Marie Curie - Paris VI, Laboratoire de cytologie, 7 Quai Saint-Bernard, 73230 Paris Cedex 05 (France)
- Commissariat a l'energie atomique - CEA, Institut de protection et de surete nucleaire - IPSN, Departement de protection sanitaire, Laboratoire de Cytogenetique Experimentale, service de Pathologie Experimentale, BP-6, 92290 Fontenay-aux-Roses (France)
Description
A method is developed, based on the incorporation of 5-Bromodeoxyuridine into the chromosomes of human lymphocytes in culture, making it possible to highlight sister chromatid exchanges and to obtain R band marking (reverse) on mitoses which have already incorporated this substance during two consecutive cycles. The method involves controlled thermal denaturation, followed by coloring with acridine orange. An application to the study of radiation-induced chromosomal damage in human lymphocytes irradiated in G0 revealed an increase and a non-random distribution of sister chromatid exchanges
Abstract (French)
Une methode basee sur l'incorporation de 5-Bromodeoxyuridine dans les chromosomes de lymphocytes humains en culture est developpee. Elle permet de mettre en evidence les echanges de chromatides soeurs et d'obtenir un marquage en bandes R (reverse) sur des mitoses ayant deja incorpore cette substance pendant deux cycles consecutifs. La methode fait intervenir une denaturation thermique menagee, suivie d'une coloration par l'acridine orange. Une application a l'etude des lesions chromosomiques radio-induites dans des lymphocytes humains irradies en G0 a revele une augmentation et une distribution non aleatoire des echanges de chromatides soeurs. (auteur)Files
54107242.pdf
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Additional details
Additional titles
- Original title (French)
- Developpement d'une methode couplee de mise en evidence des echanges de chromatides soeurs et de marquage chromosomique: application a la detection de lesions chromosomiques radio-induites dans les lymphocytes humains
Publishing Information
- Imprint Pagination
- 28 p.
- Report number
- INIS-FR--23-1179
INIS
- Country of Publication
- France
- Country of Input or Organization
- France
- INIS RN
- 54107242
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Resource subtype / Literary indicator
- Thesis
- Descriptors DEI
- ACRIDINE ORANGE; BIOCHEMICAL REACTION KINETICS; BLOOD; BUDR; CELL CULTURES; CHROMOSOMES; DEOXYURIDINE; DNA DAMAGES; FRANCE; GENETIC RADIATION EFFECTS; LYMPHOCYTES; MITOSIS; SISTER CHROMATID EXCHANGES; TRACER TECHNIQUES
- Descriptors DEC
- ACRIDINES; AMINES; ANIMAL CELLS; ANTIMETABOLITES; AROMATICS; AZAARENES; AZINES; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BLOOD; BLOOD CELLS; BODY FLUIDS; BROMOURACILS; CELL DIVISION; CHROMOSOMAL ABERRATIONS; CONNECTIVE TISSUE CELLS; DEVELOPED COUNTRIES; DRUGS; DYES; EUROPE; GENETIC EFFECTS; HETEROCYCLIC COMPOUNDS; HYDROCARBONS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; LEUKOCYTES; MATERIALS; MUTATIONS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC BROMINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIDINES; PYRIMIDINES; RADIATION EFFECTS; REACTION KINETICS; RIBOSIDES; SOMATIC CELLS; URACILS; WESTERN EUROPE
Optional Information
- Notes
- 29 refs.; Available from the INIS Liaison Officer for France, see the INIS website for current contact and E-mail addresses