Nanoparticles for magnetic biosensing systems

The further development of magnetic biosensors requires a better understanding of the interaction between living systems and magnetic nanoparticles (MNPs). We describe our experience of fabrication of stable ferrofluids (FF) using electrostatic or steric stabilization of iron oxide MNPs obtained by laser target evaporation. Controlled amounts of FF were used for in vitro experiments with human mesenchymal stem cells. Their morphofunctional responses in the Fe concentration range 2–1000 maximum tolerated dose revealed no cytotoxicity. - Highlights: • Stable ferrofluids (FF) were obtained using electrostatic or steric stabilization of iron oxide MNPs obtained by laser target evaporation. • Controlled amounts of FF were used for in vitro experiments with human mesenchymal stem cells. • Cells morphofunctional response in the Fe concentration range 2–1000 maximum tolerated dose revealed no cytotoxicity. • The results can be used for magnetic biosensoring, as well as for an addressed design of cell delivery systems.