Effect of environmental stress on radiation response of Saccharomyces cerevisiae

We have investigated the effect of pH shock and oxidative stress (H2O2 effect) both separately and together on the response of Saccharomyces cerevisiae exposed to UV and gamma radiations for one hour. Exposure to these environmental stresses resulted in S. cerevisiae cells acquiring resistance to UV radiation. Presence of cycloheximide (a know protein synthesis inhibitor) during stress inhibited the acquired UV resistance. The increased UV resistance is apparently mediated through nucleotide excision repair as the stress exposure to rad3 mutants (defective in nucleotide excision repair) do not have any effect on UV response. Both types of stresses used probably follow the same path of induction of radioresistance as the effect of both of them is nonadditive. In the strains used in our study stress exposure dose not have any significant effect on gamma radiation response. (author)