Published January 2005 | Version v1
Journal article Metadata-only

Determination of the elemental composition of cyanobacteria cells and cell fractions by atomic emission and atomic absorption spectrometry

Creators

  • 1. Inst. Geokhimii i Analiticheskoj Khimii im. V.I. Vernadskogo Rossijskoj Akademii Nauk, Moscow (Russian Federation)
  • 2. Moskovskij Gosudarstvennyj Univ. im. M.V. Lomonosova, Biologicheskij Fakul'tet, Moscow (Russian Federation)

Description

An approach to studying the elemental composition of cyanobacteria Spirulina platensis and Nostoc commune using a set of complementary analytical methods (ICP-AES, PAAS, and ETAAS) was proposed . The procedures were adapted for the determination of macro- and microelements (Na, K, Mg, Ca, Fe, Mn, Cu, Mo, Zn, B, and Se) in the biomass of cyanobacteria and separated cell fractions (chloroform and water-methanol extracts and precipitates). The conditions for the mineralization of biological materials were optimized for autoclave and microwave sample preparation procedures. The evaporation and atomization of Se and Mo in a graphite furnace in the presence of chloroform and methanol were studied

Abstract (Russian)

Предложен подход к исследованию элементного состава цианобактерий Spirulina platensis и Nostoc commune. Использован комплекс взаимодополняющих аналитических методов (AЭС-ИСП, ПААС, ЭТААС). Методики адаптированы для определения макро- и микроэлементов (Na, K, Mg, Ca, Fe, Mn, Cu, Mo, Zn, B, Se) в биомассе цианобактерий и выделенных клеточных фракций (хлороформная, водно-метанольная, осадок). Оптимизированы условия минерализации биологических материалов при автоклавном и СВЧ-способах пробоподготовки. Изучены процессы испарения и атомизации Se и Mo в графитовой печи в присутствии хлороформа и метанола

Additional details

Additional titles

Original title (Russian)
Определение элементного состава клеток цианобактерий и отдельных клеточных фракций методами атомно-эмиссионной и атомно-абсорбционной спектрометрии

Publishing Information

Journal Title
Zhurnal Analiticheskoj Khimii
Journal Volume
60
Journal Issue
1
Journal Page Range
p. 35-40
ISSN
0044-4502
CODEN
ZAKHA8

INIS

Country of Publication
Russian Federation
Country of Input or Organization
Russian Federation
INIS RN
37106619
Subject category
S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
Descriptors DEI
ABSORPTION SPECTROSCOPY; ACCURACY; BACTERIA; BORON COMPOUNDS; CYANOBACTERIA; EMISSION SPECTROSCOPY; MOLYBDENUM COMPOUNDS; QUANTITATIVE CHEMICAL ANALYSIS; SAMPLE PREPARATION; SENSITIVITY
Descriptors DEC
CHEMICAL ANALYSIS; MICROORGANISMS; REFRACTORY METAL COMPOUNDS; SPECTROSCOPY; TRANSITION ELEMENT COMPOUNDS

Optional Information

Notes
14 refs., 3 figs., 2 tabs.