Hypoxia-induced LncRNA PCGEM1 promotes invasion and metastasis of gastric cancer through regulating SNAI1
Creators
- 1. Liaoning Province Cancer Hospital and Institute (Cancer Hospital of China Medical University), Gastric Cancer Department (China)
- 2. Sheng Jing Hospital of China Medical University, Pancreatic and Thyroid Surgery Department (China)
- 3. Sheng Jing Hospital of China Medical University, Emergency Department (China)
Description
Purpose
Hypoxia is an indispensable factor in the progression of metastasis. Hypoxia inducible factor-1α (HIF-1α), the core element in generating the hypoxia response, induces invasion and metastasis by promoting epithelial–mesenchymal transition (EMT). This study explored the underlying mechanism of hypoxia associated with the invasion and metastasis of gastric cancer (GC).Methods
Six methods were employed to assess the function of the long noncoding RNA (lncRNA) prostate cancer gene expression marker 1 (PCGEM1) including gene silencing, RT-PCR, the separation of nuclear and cytoplasmic fractions, scrape motility assay, transwell migration assay, and Western-blot.
Results
LncRNA PCGEM1 was overexpressed in GC cells and tissues, and was induced by hypoxia in GC cells. Additional experiments confirmed that the knockdown of PCGEM1 significantly repressed the invasion and metastasis of GC cells. SNAI1, a key transcription factor of EMT, was regulated by PCGEM1. Overexpression of SNAI1 rescued the inhibition of PCGEM1-knockdown during the invasion and metastasis of GC cells. In addition, PCGEM1 and SNAI1 jointly affected the biomarkers of EMT.
Conclusion
Our findings indicated that PCGEM1 is a hypoxia-responsive lncRNA, and contributes to the invasion and metastasis of GC. The potential mechanism is attributed to the regulation of EMT by PCGEM1 and its influence on the expression of SNAI1.
Additional details
Identifiers
Publishing Information
- Journal Title
- Clinical and Translational Oncology (Print)
- Journal Volume
- 21
- Journal Issue
- 9
- Journal Page Range
- p. 1142-1151
- ISSN
- 1699-048X
INIS
- Country of Publication
- Spain
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 54103269
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- ANIMAL TISSUES; ANOXIA; BIOLOGICAL MARKERS; GENES; INHIBITION; METASTASES; NEOPLASMS; POLYMERASE CHAIN REACTION; PROSTATE; RNA; TRANSCRIPTION FACTORS
- Descriptors DEC
- BODY; DISEASES; GENE AMPLIFICATION; GLANDS; MALE GENITALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PROTEINS
Optional Information
- Copyright
- Copyright (c) 2019 Federaci#Latin Small Letter O With Acute#n de Sociedades Espa#Latin Small Letter N With Tilde#olas de Oncolog#Latin Small Letter I With Acute#a (FESEO)