Role of the mRNA export factor Sus1 in oxidative stress tolerance in Candida albicans

Highlights: • Sus1 is required for mRNA nuclear export in Candida albicans. • SUS1 mutant showed hyper-sensitivity to oxidative stress. • Export of the OSR mRNA is associated with the activity of ROS scavenging system. • Virulence of Candida albicans was attenuated in sus1Δ/Δ. In eukaryotes, the nuclear export of mRNAs is essential for gene expression. However, little is known about the role of mRNA nuclear export in the important fungal pathogen, Candida albicans. In this study, we identified C. albicans Sus1, a nucleus-localized protein that is required for mRNA export. Interestingly, the sus1Δ/Δ displayed hyper-sensitivity to extracellular oxidative stress, enhanced ROS accumulation and severe oxidative stress-related cell death. More strikingly, although the mutant exhibited normal activation of the expression of oxidative stress response (OSR) genes, it had attenuated activity of ROS scavenging system, which may be attributed to the defect in OSR mRNA export in this mutant. In addition, the virulence of the sus1Δ/Δ was seriously attenuated. Taken together, our findings provide evidence that the mRNA export factor Sus1 plays an important role in oxidative stress tolerance and pathogenesis.