Mycosynthesis of silver nanoparticles using extract of endophytic fungi, Penicillium species of Glycosmis mauritiana , and its antioxidant, antimicrobial, anti-inflammatory and tyrokinase inhibitory activity

Silver nanoparticles were synthesized using endophytic fungal species, Penicillium species from Glycosmis mautitiana . Phytochemicals, namely tannins, saponins, terpenoids and flavonoids, were identified in Penicillium species extracts, and act as agents of reducing and capping in the conversion of silver nanoparticles into nanoparticles. Using SEM, UV-spectroscopy and XRD, the Penicillium species silver nanoparticles (PsAgNPs) were characterized. The PsAgNPs are shown to be strong antioxidants (DDPH and FRAP), have demonstrated anti-inflammatory properties by three different methods in vitro and strongly inhibited the activity of xanthine oxidase, lipoxygenase and tyrosine kinase. E. coli and P. aeruginosa bacterial species were strongly inhibited by PsAgNPs activity at maximum levels and SEM picture of P. aeruginosa confirms these effects and that they were shrunken due to the toxic effect of PsAgNPs. (paper)