Development of kit for immunoradiometric assay of prostate specific antigen

Development and optimization of a total PSA IRMA are described here. A matched pair of anti-PSA monoclonal antibodies and pure PSA were procured from a commercial source. PSA standards were prepared and calibrated with the help of a commercial kit. The tracer MAb was labelled with 125I and purified to obtain highly pure tracer (>98%) with low non-specific binding (0.2%), good (∼80%) maximum binding and long shelf life of at least 8 weeks. The capture MAb was immobilized on polystyrene tubes at 2 μg/tube and an assay was developed for the measurement of total PSA in serum with these reagents. A good correlation was observed between sample values estimated with the developed kit and the commercial kit from CIS-Bio International. (author)