Published 2012 | Version v1
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Determination of the mechanism of early endocytosis of Clostridium perfringens phospholipase C in two fibroblast cell lines

Creators

Description

The mechanism of early endocytosis of Clostridium perfringens phospholipase C toxin (Cp-PLC) was determined in Don Q cell lines (Chinese hamster lung fibroblasts) and HSF (primary explant of human skin fibroblasts). The mechanism of entry of the Cp-PLC toxin into Don Q fibroblasts through of mediated endocytosis caveolin and/or clathrin was determined by immunofluorescence. The mechanism of entry of the Cp-PLC toxin into HSF fibroblasts through of caveolin-mediated endocytosis was determined by immunofluorescence. The binding of the Cp-PLC toxin to the membrane of Don Q fibroblasts even at 4 degrees celsius was determined by immunofluorescence. The preparations were observed under a fluorescence microscope and then under a confocal microscope to confirm the placement of the toxin with caveolin or clathrin. At least 25 photographs were taken by sheet at the point of greatest colocalization

Availability note (English)

Available from Biblioteca Luis Demetrio Tinoco, Universidad de Costa Rica

Abstract (Spanish)

El mecanismo de endocitosis temprana de la toxina fosfolipasa C de Clostridium perfringens (Cp-PLC) fue determinado en las lineas celulares Don Q (fibroblastos de pulmon de hamster chino) y HSF (fibroblastos de explante primario de piel humana). El mecanismo de entrada de la toxina Cp-PLC a los fibroblastos Don Q por medio de endocitosis mediada por caveolina y/o claratina fue determinado mediante inmunofluorescencia. El mecanismo de entrada de la toxina Cp-PLC a los fibroblastos HSF por medio de endocitosis mediada por caveolina fue determinado mediante inmunofluorescencia. La union de la toxina Cp-PLC a la membrana de los fibroblastos Don Q aun a 4 grados centigrados fue determinada mediante inmunofluorescencia. Las preparaciones fueron observadas en un microscopio de fluorescencia y posteriormente en el microscopio confocal para confirmar la colocalizacion de la toxina con caveolina o clatrina. Un minimo de 25 fotografias fueron tomadas por lamina en el punto de mayor colocalizacion

Additional details

Additional titles

Original title (Spanish)
Determinacion del mecanismo de endocitosis temprana de fosfolipasa C de Clostridium perfringens en dos lineas celulares de fibroblastos

Publishing Information

Imprint Place
San Jose (Costa Rica)
Imprint Pagination
50 p.

INIS

Country of Publication
Costa Rica
Country of Input or Organization
Costa Rica
INIS RN
50018437
Subject category
S60: APPLIED LIFE SCIENCES; S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Thesis, Non-conventional Literature
Descriptors DEI
CELL MEMBRANES; CLOSTRIDIUM PERFRINGENS; COMPARATIVE EVALUATIONS; FIBROBLASTS; FLUORESCENCE; IMMUNOASSAY; MEMBRANE PROTEINS; MICROSCOPES; TOXINS; TRACER TECHNIQUES
Descriptors DEC
ANIMAL CELLS; ANTIGENS; BACTERIA; BIOASSAY; CELL CONSTITUENTS; CLOSTRIDIUM; CONNECTIVE TISSUE CELLS; EMISSION; EVALUATION; HAZARDOUS MATERIALS; ISOTOPE APPLICATIONS; LUMINESCENCE; MATERIALS; MEMBRANES; MICROORGANISMS; ORGANIC COMPOUNDS; PHOTON EMISSION; PROTEINS; SOMATIC CELLS; TOXIC MATERIALS

Optional Information

Notes
Tab., figs., refs.