Published August 2003 | Version v1
Journal article Metadata-only

Structural analysis of γ radiation-induced chromosomal aberrations observed by atomic force microscopy

Creators

  • 1. Academy of Military Medical Sciences, Beijing (China). Inst. of Radiation Medicine

Description

Objective: To find a new method for the measurement of radiation-induced damage, the structures of normal chromosomes and 60Co γ-ray-induced chromosomal aberration were analyzed by atomic force microscopy. Methods: Normal and irradiated chromosomes of human peripheral blood lymphocytes were prepared, then three-dimensional structure and height of chromosomes were analyzed by atomic force microscopy. Results: Three-dimensional structures of normal chromosomes and dicentric aberration in irradiated chromosomes were observed clearly. The data of chromosome height were helpful to recognizing the dicentric aberrations. Conclusion: Atomic force microscopy providing three-dimension image and linear measurement is a new and valuable tool for structural analysis of radiation-induced chromosomal aberrations

Additional details

Publishing Information

Journal Title
Chinese Journal of Radiological Medicine and Protection
Journal Volume
23
Journal Issue
4
Journal Page Range
p. 263-264
ISSN
0254-5098

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
36021297
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Numerical Data
Descriptors DEI
ATOMIC FORCE MICROSCOPY; BLOOD; CELL CULTURES; CHROMOSOMAL ABERRATIONS; COBALT 60; DICENTRIC CHROMOSOMES; DOSE RATES; EXPERIMENTAL DATA; GAMMA RADIATION; IMAGE PROCESSING; LYMPHOCYTES; RADIATION DOSES; RADIATION INJURIES; RESOLUTION
Descriptors DEC
ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BLOOD; BLOOD CELLS; BODY FLUIDS; CHROMOSOMES; COBALT ISOTOPES; CONNECTIVE TISSUE CELLS; DATA; DISEASES; DOSES; ELECTROMAGNETIC RADIATION; INFORMATION; INJURIES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LEUKOCYTES; MATERIALS; MICROSCOPY; MINUTES LIVING RADIOISOTOPES; MUTATIONS; NUCLEI; NUMERICAL DATA; ODD-ODD NUCLEI; PROCESSING; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; SOMATIC CELLS; YEARS LIVING RADIOISOTOPES