Published August 1998
| Version v1
Journal article
A New Experiment for the Measurement of nJ(C,P) Coupling Constants Including 3J(C4'i,Pi) and 3J(C4'i,Pi+1) in Oligonucleotides
Creators
- 1. Universitaet Frankfurt, Institut fuer Organische Chemie (Germany)
- 2. Center for Advanced Research in Biotechnology (United States)
Description
A new experiment for the measurement of nJ(C,P) coupling constants along the phosphodiester backbone in RNA and DNA based on a quantitative-J HCP experiment is presented. In addition to coupling constants, in which a carbon atom couples to only one phosphorus atom, both the intraresidual 3J(C4'i,Pi) and the sequential 3J(C4'i,Pi+1) for the C4' resonances that couple to two phosphorus atoms can be obtained. Coupling constants obtained by this new method are compared to values obtained from the P-FIDS experiment. Together with 3J(H,P) coupling constants measured using the P-FIDS experiment, the backbone angles β and element of can be determined
Additional details
Identifiers
Publishing Information
- Journal Title
- Journal of Biomolecular NMR
- Journal Volume
- 12
- Journal Issue
- 2
- Journal Page Range
- p. 223-230
- ISSN
- 0925-2738
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 39105447
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- COUPLING CONSTANTS; HCP LATTICES; OLIGONUCLEOTIDES; PHOSPHORUS; RNA
- Descriptors DEC
- CRYSTAL LATTICES; CRYSTAL STRUCTURE; DNA; ELEMENTS; HEXAGONAL LATTICES; NONMETALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS
Optional Information
- Copyright
- Copyright (c) 1998 Kluwer Academic Publishers