Preparation of sodium 2-mercapto-[14C]ethanesulfonate

Starting from [1-14C]ethanol, 1,2-dibromo-[14C]-ethane was prepared by a usual method through [14C]-ethylene. Sodium sulfite was alkylated by 1,2-dibrom-[14C]ethane and the formed sodium 2-brom-[14C]ethane sulfonate was transferred without isolation to a labelled 2-[(aminoiminomethyl)thio]-[14C]ethanesulfonic acid. After purification by crystallization of the acid, 2-mercapto-[14C]ethanesulfonic acid was prepared through guanidinium salt. After the neutralization of 2-mercapto-[14C]ethanesulfonic acid by sodium hydroxide, the corresponding sodium salt with specific activity 0.083 μCi/mmol (0.31 MBq/mmol) was prepared. (author)