Accumulation, transformation and subcellular distribution of arsenite associated with five carbon nanomaterials in freshwater zebrafish specific-tissues

Highlights: • GO and GN promoted As toxicity, while SWCNT, MWCNT and C₆₀ had a negligible effect. • GN and GO influenced As accumulation and species transformation. • As accumulation correlated linearly with intestinal As(V), As(III) and MMA levels. • In the intestine, most As was distributed in MTLPs in the form of MMA. • In the muscle and head, most As was distributed in cellular debris as DMA and AsB. Although carbon nanomaterials (CNMs) commonly exist throughout the aquatic environment, their effect on arsenic (As) distribution and toxicity is unclear. In this study, arsenite accumulation, transformation, subcellular distribution, and enzyme activity were assessed in adult zebrafish (Danio rerio) intestines, heads and muscles, following co-exposure to arsenite and CNMs with different structures (single-walled carbon nanotubes (SWCNTs), multi-walled carbon nanotubes (MWCNTs), fullerene (C₆₀), graphene oxide (GO), and graphene (GN)). Results show that GN and GO promoted As toxicity in D. rerio, as carriers increasing total As accumulation in the intestine, resulting in arsenite adsorbed by GO and GN being released and transformed mainly into moderately-toxic monomethylarsonic acid (MMA), which was mostly distributed in organelles and metallothionein-like proteins (MTLPs). Moreover, GO and GN influenced As species distribution in D. rerio due to the excellent electron transfer ability. However, the effect was marginal for SWCNT, MWCNT and C₆₀, because of the different structure and suspension stability in fish-culture water. In addition, in the muscle and head tissues, As was mainly distributed in cellular debris in the forms of dimethylarsinic acid (DMA) and arsenobetaine (AsB). These findings help better understand the influence of CNMs on the mechanism of As toxicity in natural aquatic environments.