Cytological and mutagenic effects of ions implantation on rice plants

After implanting 75,100 and 150 keV of N+ ion and 20 keV of N+, H+ and Ar+ ions of various doses to rice seeds, the cytological (in M1) and mutagenic (in M2) effects were studied. The results indicated that all the methods could induce the variation of the chromosome structure in rice plant. the rate of cells with chromosomal aberration tended to increase with the ion implanting energy and dose. However, the rate of cells with chromosomal aberration was lower than that induced with 60Co γ-rays. Frequency of the chlorophyll mutation and useful mutation induced by implantation of N+, H+ and Ar+ ions were higher than those induced by γ-rays