Exposure to lethal levels of benzo[a]pyrene or cadmium trigger distinct protein expression patterns in earthworms (Eisenia fetida)

Highlights: • Different pollutants induce distinct patterns of protein expression in earthworms. • mRNA expression does not correlate well with protein expression. • Metallothionein gene expression was specifically induced by the Cd treatment. • Worms subjected to the BaP or Cd treatments exhibited opposite patterns of CAT expression. - Abstract: Different pollutants induce distinct toxic responses in earthworms (Eisenia fetida). Here, we used proteomics techniques to compare the responses of E. fetida to exposure to the 10% lethal concentration (14d-LC₁₀) of benzo[a]pyrene (BaP) or cadmium (Cd) in natural red soil (China). BaP exposure markedly induced the expression of oxidation-reduction proteins, whereas Cd exposure mainly induced the expression of proteins involved in transcription- and translation-related processes. Furthermore, calmodulin-binding proteins were differentially expressed upon exposure to different pollutants. The calcium (Ca²⁺)-binding cytoskeletal element myosin was down-regulated upon BaP treatment, whereas the Ca²⁺-binding cytoskeletal element tropomyosin-1 was up-regulated upon Cd treatment. Some proteins exhibited opposite responses to the two pollutants. For instance, catalase (CAT) and heat shock protein 70 were up-regulated upon BaP treatment and down-regulated upon Cd treatment. A significant (p < 0.05, one-way ANOVA with least-significant difference (LSD) test) increase in the level of reactive oxygen species (ROS) and CAT activity further showed that BaP mainly induces oxidative stress. Real-time PCR analysis showed that mRNA expression often did not correlate well with protein expression in earthworms subjected to Cd or BaP treatment. In addition, the expression of the gene encoding the protein metallothionein, which was not detected in the protein analysis, was induced upon Cd treatment, but slightly reduced upon BaP treatment. Therefore, BaP and Cd have distinct effects on the protein profile of E. Fetida with BaP markedly inducing ROS activity, and Cd mainly triggering genotoxicity. Capsule summary: Distinct patterns of protein expression are induced in earthworms upon exposure to different pollutants; BaP markedly induces high levels of ROS, while Cd resultes in genotoxicity.