Published June 1988
| Version v1
Journal article
Preparative HPLC separation of glycocholic acid-(125I-histamine)
- 1. Academia Sinica, Beijing, BJ (China). Inst. of Atomic Energy
Description
A preparative HPLC separation method of glycocholic acid-(125I-histamine) is described. The separation was carried out on YWG-CH reverse phase column by using methanol:isopropanol:water (60:5:40,V/V) as mobile phase. THe separation was 30 min. The flow rate was 1ml/min. Using this method, glycocholic acid-(125I-histamine) was purified and satisfactorily used for RIA. The radiochemical purity was 98%. The recovery efficiency was 99.1%
Additional details
Publishing Information
- Journal Title
- Journal of Isotopes
- Journal Volume
- 1
- Journal Issue
- 1
- Series
- J. Isot.
- Journal Page Range
- 44, 45-47
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 20075004
- Subject category
- S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
- Descriptors DEI
- CHOLIC ACID; GLYCINE; HISTAMINE; IODINE 125; LABELLED COMPOUNDS; LABELLING; LIQUID COLUMN CHROMATOGRAPHY; PURIFICATION
- Descriptors DEC
- AMINES; AMINO ACIDS; AZOLES; BETA DECAY RADIOISOTOPES; BILE ACIDS; CARBOXYLIC ACIDS; CHROMATOGRAPHY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; IMIDAZOLES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; RADIOISOTOPES; SEPARATION PROCESSES; STEROIDS; STEROLS