Published December 1984
| Version v1
Journal article
Modified radiometric assay for 3-hydroxy-3-methylglutaryl coenzyme a reductase
Description
A radiometric assay for measuring the activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is described. The assay is based on the separation of the mevalonate product from HMG-CoA by high-voltage electrophoresis. This method is more sensitive and more specific than the NADPH-based spectrophotometric assay, and less tedious than available radiometric assays. It has been used to measure HMG-CoA reductase activity in crude extracts of Saccharomyces cerevisiae and in human skin fibroblasts
Additional details
Publishing Information
- Journal Title
- Lipids
- Journal Volume
- 19
- Journal Issue
- 12
- Series
- Lipids.
- Journal Page Range
- 966-970
- ISSN
- 0024-4201
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 16084921
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- CATALYSIS; CELL CULTURES; ELECTROPHORESIS; ENZYMES; FIBROBLASTS; METABOLISM; OXIDOREDUCTASES; RADIOMETRIC ANALYSIS; SACCHAROMYCES CEREVISIAE; SKIN
- Descriptors DEC
- ANIMAL CELLS; BODY; CHEMICAL ANALYSIS; CONNECTIVE TISSUE CELLS; FUNGI; MICROORGANISMS; ORGANIC COMPOUNDS; ORGANS; PLANTS; QUANTITATIVE CHEMICAL ANALYSIS; SACCHAROMYCES; SOMATIC CELLS; YEASTS