Preliminary gene mapping for anther culture ability based on a new wheat germplasm with high anther culture ability

In order to excavating QTLS velated to anther culture ability and elucidating the genetic control basis of anther culture in vitro. The recombinant inbred lines containing 113 progenies were derived from the cross of H307 γ-rays induced mutant and elite cultivar Zheng 9023, which was an early maturing variety with high-quality that widely applied in the production. The results of phenotypic analysis revealed that two-way transgressive segregation were observed in the traits of callus induction frequency, green plantlet regeneration frequency, albino plantlet differentiation frequency, and albino plantlet yield frequency. Whereas, green plantlet yield frequency was obvious one-way transgressive segregation with low-parent. The simple sequence repeat (SSR) molecular marker technology and inclusive composite interval mapping (ICIM) in QTL Icimapping 3.3 software were applied for QTL mapping of anther culture ability associated gene markers. A total of four QTLs loci related to the traits of callus induction frequency, green plantlet yield frequency and albino plantlet differentiation frequency were detected, which were located on 5 B, 6 A and 3 D chromosome, respectively. Additionally, the QTL on 6 A chromosome is possible to be a new locus associated with green plantlet yield frequency and the additive effect allele of this QTL was come from H307. The results of this study may lay a foundation for overcoming the limitation of genotype dependence in anther culture. (authors)