Cytological effects induced by 60Co γ-irradiation on moso seed

In this experiment, Moso seeds were irradiated by different doses of 60Co γ rays and the effects on chromosomal aberration, nuclear aberration and mitosis of the root tip cells of were studied. The results indicated that γ-rays irradiation with lower dosed could promote and stimulate mitosis of root tip cells, and with high doses could inhibit mitosis of root tip cell. Furthermore, γ-rays irradiation could induce many types of chromosomal and nuclear aberration, such as chromosome bridges, chromosome fragment, laggard chromosome (clump), free chromosome (clump), chromosome conglutination, micronucleus, minornucleus, double nucleus, nuclear budding, nuclear bled, nuclear fragmentation and so on. The relationship between dosages and frequencies of chromosomal and nuclear aberration of root tip cells was fitted in the model of Y = 0.0390X-0.03756, Y = 0.0530X-0.0783, respectively. The correlation coefficient was 0.8934 and 0.9982. The relationship between frequency of chromosomal aberration and frequency of Moso micronucleus of root tip cell was fitted in with the model Y = 0.8836X-0.4525. The correlation coefficient was 0.8345, F =25.22> F0.01. (authors)