Influence of radioprotective agents on the activities of isoenzymes of blood serum alkaline phosphatase in irradiated dogs
- 1. Karlova Universita, Hradec Kralove (Czecheslovakia). Dept. of Biochemistry
- 2. Ceska Lekarska Spolecnost J.E. Purkyne, Hradec Kralove (Czechoslovakia)
Description
Changes of the total serum activity of alkaline phosphatase (AP) and its bone and intestinal isoenzymes were studied in dogs γ-irradiated by 3.0 Gy. The activities of AP isoenzymes were determined by means of a heat inactivation-inhibition method. After irradiation the serum AP activities were lower in general. In case of the bone isoenzyme the decrease was most pronounced. The changes of the total AP activity and of the intestinal isoenzyme were less distinct. The i.m. administration of the radioprotective mixture cystamine with mexamine (24 mg/kg + 4 mg/kg) before irradiation led only to a moderate reduction of the radiation-altered AP values in the dogs. The present results indicate that the radioprotective way used here renders only an unsatisfactory protection for large laboratory animals against ionizing radiation. (orig.)
Abstract (German)
Die Veraenderungen in der Gesamtaktivitaet der alkalischen Phosphatase (AP) und ihrer Knochen- und Darmisoenzyme im Blutserum wurden an Hunden nach Gammabestrahlung mit 0,3 Gy untersucht. Die Aktivitaet wurde mit Hilfe der Thermoinaktivations-Inhibitionsmethode bestimmt. Nach der Bestrahlung wurde eine erniedrigte Aktivitaet der Serum-AP gefunden, die im Falle des Knochenisoenzyms am deutlichsten ausgepraegt war. Die Veraenderungen der Gesamtaktivitaet und des Darmisoenzyms waren weniger auffallend. Die intramuskulaere Verabreichung der radioprotektiven Kombination Cystamin mit Mexamin (24 mg/kg + 4 mg/kg) vor der Bestrahlung bewirkte bei Hunden nur eine maessige Verbesserung der strahlungsbedingt veraenderten AP-Werte. Die Ergebnisse zeigen, dass der benutzte Strahlenschutz bei grossen Versuchstieren nur eine ungenuegende Protektion gegen die ionisierende Strahlung bietet. (orig.)Additional details
Publishing Information
- Journal Title
- Strahlentherapie
- Journal Volume
- 156
- Journal Issue
- 5
- Series
- Strahlentherapie.
- Journal Page Range
- 365-367
- ISSN
- 0039-2073
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 12581397
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ALKALINE PHOSPHATASE; BEAGLES; BLOOD SERUM; CATALYSIS; CYSTAMINE; GAMMA RADIATION; IRRADIATION; ISOENZYMES; LOCAL RADIATION EFFECTS; METABOLISM; MEXAMINE; RADIATION PROTECTION
- Descriptors DEC
- AMINES; ANIMALS; AZOLES; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; DOGS; DRUGS; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERASES; ETHERS; HETEROCYCLIC COMPOUNDS; HYDROLASES; HYDROXY COMPOUNDS; INDOLES; IONIZING RADIATIONS; MAMMALS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PHOSPHATASES; PYRROLES; RADIATION EFFECTS; RADIATIONS; RADIOPROTECTIVE SUBSTANCES; RESPONSE MODIFYING FACTORS; SEROTONIN; SYMPATHOMIMETICS; TRYPTAMINES; VERTEBRATES