Production of L-[1-/sup 11/C]valine by HPLC resolution
- 1. Oak Ridge Associated Universities, Tennessee
Description
Based on a recently developed analytical technique, preparative high-performance liquid chromatographic (HPLC) resolution of DL-[1-/sup 11/C]valine has been achieved. A conventional reverse-phase HPLC column and a chiral mobile phase (aqueous solution of L-proline, cupric acetate, and sodium acetate) were used. The copper can be removed from the L-valine fraction by precipitation as the sulfide, and final purification by cation-exchange chromatography yields L-[1-/sup 11/C]valine in a form that is acceptable for clinical positron tomographic studies. This purification method does not remove the L-proline introduced in the resolution process, but added L-proline did not affect the tissue distribution of L-[1-/sup 14/C]valine in rats. We have produced up to 60 mCi of L-[1-/sup 11/C]valine in an overall synthesis and resolution time of 50 min. This procedure should be adaptable to the rapid resolution of other C-/sup 11/-labeled amino acid racemates
Additional details
Publishing Information
- Journal Title
- J. Nucl. Med.
- Journal Volume
- 23
- Journal Issue
- 1
- Series
- J. Nucl. Med.
- Journal Page Range
- 29-33
- ISSN
- 0022-3123
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 13708336
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE; S62: RADIOLOGY AND NUCLEAR MEDICINE; S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
- Descriptors DEI
- CARBON 11; CHEMICAL PREPARATION; DIAGNOSIS; LIQUID COLUMN CHROMATOGRAPHY; POSITRON COMPUTED TOMOGRAPHY; RADIOPHARMACEUTICALS; VALINE
- Descriptors DEC
- AMINO ACIDS; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; CARBON ISOTOPES; CARBOXYLIC ACIDS; CHROMATOGRAPHY; COMPUTERIZED TOMOGRAPHY; DIAGNOSTIC TECHNIQUES; DRUGS; EMISSION COMPUTED TOMOGRAPHY; EVEN-ODD NUCLEI; ISOTOPES; LABELLED COMPOUNDS; LIGHT NUCLEI; MINUTES LIVING RADIOISOTOPES; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; RADIOACTIVE MATERIALS; RADIOISOTOPES; SEPARATION PROCESSES; SYNTHESIS; TOMOGRAPHY