Published March 21, 2008
| Version v1
Journal article
Structure of the cold-shock domain protein from Neisseria meningitidis reveals a strand-exchanged dimer
Creators
- 1. Division of Structural Biology, Henry Wellcome Building for Genomic Medicine, University of Oxford, Roosevelt Drive, Oxford OX3 7BN (United Kingdom)
- 2. The Oxford Protein Production Facility, Henry Wellcome Building for Genomic Medicine, University of Oxford, Roosevelt Drive, Oxford OX3 7BN (United Kingdom)
- 3. Bacterial Pathogenesis and Functional Genomics Group, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE (United Kingdom)
Description
The X-ray crystal structure of the cold-shock domain protein from N. meningitidis reveals a strand-exchanged dimer. The structure of the cold-shock domain protein from Neisseria meningitidis has been solved to 2.6 Å resolution and shown to comprise a dimer formed by the exchange of two β-strands between protein monomers. The overall fold of the monomer closely resembles those of other bacterial cold-shock proteins. The neisserial protein behaved as a monomer in solution and was shown to bind to a hexathymidine oligonucleotide with a stoichiometry of 1:1 and a Kd of 1.25 µM
Availability note (English)
Available from http://dx.doi.org/10.1107/S1744309108005411; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374261Additional details
Identifiers
- URL
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374261;
- DOI
- 10.1107/S1744309108005411;
- PII
- S1744309108005411;
Publishing Information
- Journal Title
- Acta Crystallographica. Section F
- Journal Volume
- 64
- Journal Issue
- Pt 4
- Journal Page Range
- p. 247-251
- ISSN
- 1744-3091
- CODEN
- ACSFCL
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 46065807
- Subject category
- S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
- Descriptors DEI
- CRYSTAL STRUCTURE; DIMERS; MATHEMATICAL SOLUTIONS; MONOMERS; PROTEINS; RESOLUTION; SOLUTIONS; STOICHIOMETRY
- Descriptors DEC
- DISPERSIONS; HOMOGENEOUS MIXTURES; MIXTURES; ORGANIC COMPOUNDS
Optional Information
- Copyright
- Copyright (c) International Union of Crystallography 2008
- Notes
- PMCID: PMC2374261; PMID: 18391418; PUBLISHER-ID: sw5023; OAI: oai:pubmedcentral.nih.gov:2374261