Published November 16, 1981 | Version v1
Journal article Metadata-only

Catecholamine levels and turnover during aging in brain regions of male C57BL/6J mice

Creators

  • 1. The Andrus Gerontology Center, Los Angeles, CA (USA)
  • 2. University of Southern California, Los Angeles (USA). Dept. of Biological Sciences

Description

A radioenzymatic assay is described for measuring brain catecholamines (CA) and 3,4-dihydroxyphenlyacetic acid (DOPAC) in the same tissue extract. The [3H]methylated products are differentially extracted and then acetylated by acetic anhydride, followed by thin-layer chromatography in non-basic solvents. Routine sensitivity is 3-5 pg per sample. This assay gave CA levels in brain regions which generally agreed with previous reports. (Auth.)

Additional details

Publishing Information

Journal Title
Brain Res.
Journal Volume
224
Journal Issue
2
Series
Brain Res.
Journal Page Range
337-352
ISSN
0006-8993

INIS

Country of Publication
Netherlands
Country of Input or Organization
Netherlands
INIS RN
13653746
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
ACETYLATION; AGE DEPENDENCE; BRAIN; CATECHOLAMINES; DOPAMINE; METABOLISM; METHYLATION; MICE; RADIOCHROMATOGRAPHY; SOLVENT EXTRACTION; TRANSFERASES; TRITIUM COMPOUNDS
Descriptors DEC
ACYLATION; AMINES; ANIMALS; AROMATICS; BODY; CENTRAL NERVOUS SYSTEM; CHEMICAL REACTIONS; CHROMATOGRAPHY; DRUGS; ENZYMES; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; MAMMALS; NERVOUS SYSTEM; ORGANIC COMPOUNDS; ORGANS; PHENOLS; POLYPHENOLS; RODENTS; SEPARATION PROCESSES; SYMPATHOMIMETICS; VERTEBRATES