Preliminary study on the time course of heavy ion-induced DNA damage in hepatoma cells
- 1. Chinese Academy of Sciences, Lanzhou (China). Inst. of Modern Physics
- 2. Lanzhou Univ., Lanzhou (China). School of Life Sciences
Description
In this paper, the time course of DNA damage in the human hepatoma cell line SMMC-7721 irradiated with 80 MeV/μ 20Ne10+ was investigated. Irradiation was performed at the Heavy Ion Research Facility in Lanzhou (HIRFL), and the single cell gel electrophoresis (SCGE) was used to detect the DNA damage. Results showed that the 'comet' tail length for the immediate irradiation-caused DNA damage had a positively linear correlation with the irradiation dose in the dose range of 0-8 Gy and the severer secondary damage was observed by 24h after irradiation. The results suggested that the heavy ion 80 MeV/μ 20Ne10+, as a high LET radiation, had stronger effect than low LET radiations on cells, although the response curves for them were all positively linear
Additional details
Publishing Information
- Journal Title
- Journal of Radiation Research and Radiation Processing
- Journal Volume
- 21
- Journal Issue
- 4
- Journal Page Range
- p. 264-267
- ISSN
- 1000-3436
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 35074448
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- APOPTOSIS; DNA DAMAGES; DOSE-RESPONSE RELATIONSHIPS; ELECTROPHORESIS; HEAVY IONS; HEPATOMAS; LET; NEON 20; NEON IONS; RADIATION DOSES; RADIATION INJURIES; TIME DEPENDENCE; TUMOR CELLS
- Descriptors DEC
- ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; CARCINOMAS; CHARGED PARTICLES; DISEASES; DOSES; ENERGY TRANSFER; EVEN-EVEN NUCLEI; INJURIES; IONS; ISOTOPES; LIGHT NUCLEI; NEON ISOTOPES; NEOPLASMS; NUCLEI; RADIATION EFFECTS; STABLE ISOTOPES