Authenticity identification of mutants induced by EMS in wheat using SSR marker and SNP chips

In order to identify the authenticity of EMS mutation, SSR markers and 90 K SNP chip were used to detect Wheat Strain H261 and its EMS mutants. SSR analysis showed that the difference of SSR markers between H261 and LF2010 or LF2099 was 0, but the difference of SSR markers between H261 and LF2100 was 10, and the polymorphism rate was 47.62%. SNP chip analysis showed that the differences between H261 and LF2010 and LF2099 were 66 and 12, accounting for 0.0809% and 0.0147% of the total SNPS, respectively. The homozygous differences between the two mutants and H261 were 0, while the differences between H261 and LF2100 were 2846, accounting for 3.4879% of the total SNPS, and the homozygous differences between them were 784, accounting for the 0.9608%. The results showed the genetic background of LF2010 and LF2099 were highly consistent with that of genetic background of parents H261, and were the mutated offspring of H261 mutated by EMS, while LF2100 was a 'pseudomutant' produced by natural outcrossing or mechanical hybridization. This study laid a theoretical foundation for the better use of wheat mutants in genetic improvement and functional genome research. (authors)